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SRX4774148: GSM3405616: Biofilms formed in LB on borosilicate glass discs 2; Pseudomonas aeruginosa; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 16.1M spots, 3.2G bases, 2Gb downloads

Submitted by: NCBI (GEO)
Study: Transcriptome Sequence of Antibiotic-Treated Pseudomonas aeruginosa
PRJNA493733 • SRP162817 • All experiments • All runs
show Abstracthide Abstract
Pseudomonas aeruginosa is known to tolerate antibiotic therapy during infection. This prevents clearance of infection and negatively impacts patient outcomes. Here, we report the transcriptome sequence of antibiotic-treated and untreated P. aeruginosa cultures and the differential gene expression observed when treated cells are compared to untreated cells. Overall design: P. aeruginosa antibiotic tolerance, biofilm, planktonic, and stationary cultures
Sample: Biofilms formed in LB on borosilicate glass discs 2
SAMN10141777 • SRS3854688 • All experiments • All runs
Organism: Pseudomonas aeruginosa PAO1
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA was isolated from untreated and persister cells using the RNeasy Kit (QIAgen) according to manufacturer's instructions and treated twice with TURBO DNase (Ambion, Grand Island, NY) to remove any contaminating DNA (5, 6). Ribosomal RNA was depleted using the Ribo-Zero Magnetic kit (Epicentre, Madison, WI) Directional library was generated using the NEXTflex directional RNA-seq kit (Bioo Scientific, Austin, TX)
Experiment attributes:
GEO Accession: GSM3405616
Links:
Runs: 1 run, 16.1M spots, 3.2G bases, 2Gb
Run# of Spots# of BasesSizePublished
SRR793898316,051,4963.2G2Gb2018-10-31

ID:
6458353

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